Question

We often find catchy slogans in laundry detergents like, with biologically active enzymes. 

(i) Identify this enzyme.

(ii) Name the residues which contributed to its enzymatic activity.

(iii) Native form of this enzyme is inactivated by bleach. Give reason.

(iv) Is there any way to retain the activity even in the presence of bleach?

Answer 1

(i) Subtilisin

(ii) Residues are Ser 221, His 64 and Asp 32 1

(iii) Oxidation of amino acid Methionine at position 222

(iv) Substitution of Met 222 by Ala using site directed mutagenesis